Affinity purification
| Product Name: | Recombinant Protein G NUPharose Fast Flo |
| Alternative Name: | ProG NUPharose FF |
| Catalog Number: | NRPB02 |
Description
Matrix ：Cross-linked agarose, 4%
Ligand ：Recombinant Protein G, ≥6 mg/mL
Particle size range： 45~165 μm
Particle size, d50a ：~90 μm
Dynamic binding capacity, DBC10% b ：≥40 mg h-IgG/mL
Recommended operating flow velocityc ：60~300 cm/h
Maximum flow velocityd ：900 cm/h, 0.3 MPa
pH stability ：3~9 (recommended operating pH), 2~10 (short-term)
Chemical stability ：Stable to common aqueous buffers: 10~100 mmol/L NaOH (CIP), 6 M guanidine hydrochloride, 70% ethanol (CIP)
Storage & Shipping ：20% ethanol, 2~8 ℃, shipping 2~30 ℃
Shelf life ：3 years
Note: aMore than 90% of the microspheres by volume are in this size range; b Dynamic binding capacit at 10% breakthrough for h-IgG with 6 min residence time; cThe flow rate can meet the use conditions of 2-6 min residence time for various types of columns; d1 cm diameter column and 10 cm bed height using buffers with the same viscosity as water at 20 °C.
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E-mail: marketing@nuptec.com
Add.(factory): No.63, Yuanwu Road, Guanli Town, Xiaoshan District, Hangzhou, Zhejiang
