Recombinant Alkali-tolerant Protein A NUPharose Fast Flow

Product Name:	Recombinant Alkali-tolerant Protein A NUPharose Fast Flow
Alternative Name:	rat-PA NUPharose FF
Catalog Number:	NRPB06

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Description

Matrix ：Cross-linked agarose, 4%

Ligand ：Recombinant alkali-resistant protein A,≥6 mg/mL

Particle size range： 45~165 μm

Particle size, d50^a ：~90 μm

Dynamic binding capacity, DBC_10% ^b ：≥45 mg h-IgG/mL

Recommended operating flow velocity^c ：60~300 cm/h

Maximum flow velocity^d：900 cm/h, 0.3 MPa

pH stability ：3~10 (recommended operating pH), 3~12 (short-term)

Chemical stability ：Stable in the following solutions: 0.1~0.5 M NaOH (CIP), 6 M guanidine hydrochloride, 70% ethanol (CIP), etc.

Storage & Shipping ：20% ethanol, 2~8 ℃, shipping 2~30 ℃

Shelf life ：3 years

Note: ^aMore than 90% of the microspheres by volume are in this size range; ^b Dynamic binding capacity at 10% breakthrough for h-IgG with 6 min residence time; ^cThe flow rate can meet the use conditions of 2-6 min residence time for various types of columns; ^d1 cm diameter column and 10 cm bed height using buffers with the same viscosity as water at 20 °C.