Protein A/G NUPharose Fast Flow

Product Name:	Protein A/G NUPharose Fast Flow
Alternative Name:	PAG NUPharose FF
Catalog Number:	NRPB13

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Description

Matrix ：Cross-linked agarose, 4%

Ligand ：Protein A-Protein G fusion protein, ≥6 mg/mL

Particle size range： 45~165 μm

Particle size, d50^a ：~90 μm

Dynamic binding capacity, DBC_10% ^b ：≥40 mg h-IgG/mL

Recommended operating flow velocity^c ：60~300 cm/h

Maximum flow velocity^d：900 cm/h, 0.3 MPa

pH stability ：3~9 (recommended operating pH), 2~10 (short-term)

Chemical stability ：Stable to common aqueous buffers: 10~100 mmol/L NaOH (CIP), 6 M guanidine hydrochloride, 70% ethanol (CIP)

Storage & Shipping ：20% ethanol, storage 2~8 ℃, shipping 2~30 ℃

Shelf life ：3 years

Note：^aMore than 90% of the microspheres by volume are in this size range；^bThe binding ability was tested by incubation for 2 hours；^c1 cm diameter column and 10 cm bed height using buffers with the same viscosity as water at 20 °C.