Recombinant Protein A NUPharose Fast Flow

Product Name:	Recombinant Protein A NUPharose Fast Flow
Alternative Name:	ProA NUPharose FF
Catalog Number:	NRPB01

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Description

Matrix：Cross-linked agarose, 4%

Ligand：Recombinant protein A (E.coli), ≥6 mg/mL

Particle size range： 45~165 μm

Particle size, d50^a ：~90 μm

Dynamic binding capacity, DBC_10%^b ： ≥40 mg h-IgG/mL

Recommended operating flow velocity^c： 60~300 cm/h

Maximum flow velocity^d： 900 cm/h, 0.3 MPa

pH stability：3~10 (recommended operating pH), 3~12 (short-term)

Chemical stability： Stable to common aqueous buffers: 10~100 mmol/L NaOH (CIP), 6 M guanidine hydrochloride, 70% ethanol (CIP)

Storage & Shipping： 20% ethanol, storage 2~8 ℃, shipping 2~30 ℃

Shelf life ：3 years

Note: ^aMore than 90% of the microspheres by volume are in this size range; ^b Dynamic binding capacity at 10% breakthrough for h-IgG with 6 min residence time; ^cThe flow rate can meet the use conditions of 2-6 min residence time for various types of columns; ^d1 cm diameter column and 10 cm bed height using buffers with the same viscosity as water at 20 °C.