Streptavidin Conjugated Horseradish Peroxidase

Product Name:	Streptavidin Conjugated Horseradish Peroxidase, Streptavidin-HRP, SA-HRP
Catalog Number:	NRPA16
Packing Details:	0.2 ml, 1 ml, 10 mg
Formulation:	liquid 1mg/ml SA-HRP, 10mM PB, 50%glycerol, pH7.4; Lyophilized from 10mM PB (8mM Na₂HPO₄, 2mM NaH₂PO₄, pH7.4)
Storage Condition:	-20℃
Storage Duration:	3 years

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Description

Description:

Streptavidin conjugated horseradish peroxidase (SA-HRP) is a cross-linked product of high-purity streptavidin (SA) and high-purity horseradish peroxidase. The application of it are detecting biotinylated antibody, protein, tissue, cell, nucleic acid and other samples, often used as a signal amplifier to improve the detection sensitivity.

Streptavidin (SA) is a tetramer protein formed by four identical subunits, with a molecular weight of about 60kDa, which have a specific binding to biotin, and one SA can bind to four biotin. Compared with avidin from egg white, SA has no glycosylation and isoelectric point of it is neutral or slightly acidic, so the non-specific binding of it is much lower than avidin’s, and the non-specific background of it is very low when used for detection.

Horseradish peroxidase (HRP) is a kind of glycoprotein containing heme with about 40 kDa molecular weight , sugar content is 18%, brown color. Incubated with substrate, HRP can produce a kind of molecular derivative labeled by coloring, fluorescence or luminescence, which makes it be widely used in the detection of molecular biology, biochemistry, immunology and other fields.

The following table is the recommended dilution ratio for conventional use. In the actual test operation, the dilution ratio of SA-HRP should be adjusted appropriately according to the specific experimental conditions. Taking the conventional Western bolt as an example, 10 ml of 1:2000 diluent is needed for each test, and only 5 μ L (5 μ g) is needed for each test.

WB	ELISA	IHC/IC	Southern/Northern	EMSA
1:2000-10000	1:2000-10000	1:200-500	1:2000-5000	1:2000-5000

WB: Western bolt; IHC: Immunohistochemistry; IC: Immunocytochemistry.

For research use only!