Immobilized Metal ion Affinity Chromatography NUPharose Fast Flow

Product Name:	Immobilized Metal ion Affinity Chromatography NUPharose Fast Flow
Alternative Name:	IMAC NUPharose FF
Catalog Number:	NRPB76

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Description

Matrix ：Cross-linked agarose, 6%

Ligand ：Nitrilotriacetic acid, precharged with ~17 μmol Ni²⁺/ml

Particle range^a： 45~165 μm

Particle size, d50 ：~90 μm

Dynamic binding capacity, DBC_10% ^b ：≥40 mg His-tag protein/mL

Recommended operating flow velocity ：60~300 cm/h

Maximum flow velocity^c：1200 cm/h, 0.3 MPa

pH stability ：3~12 (recommended operating, pH), 3~12 (long-term), 2~14 (short-term)^d

Chemical stability ：Stable to common aqueous buffers: 1 M NaOH, 8 M urea, 6 M guanidine hydrochloride, 70% ethanol. The effects of common reducing agents, chelating agents, detergents, denaturants, etc. on resins are shown in the table below.

Storage & Shipping ：20% ethanol, 2~30 ℃

Shelf life ：5 years

Note: ^aMore than 90% of the microspheres by volume are in this size range; ^bDynamic binding capacity at 10% breakthrough for E. coli expressed recombinant Staphylococcus aureus protein A with a residence time of 6 mins; ^c1 cm diameter column and 10 cm bed height using buffers with the same viscosity as water at 20 °C; ^dpH stability during CIP process after stripping of metal ions.