Recombinant Streptavidin

Mol. Wt.: Tetramer: 62.4 Monomer:16.5 kDa

Resources: Escherichia coli (E. coli)

Purity: ≥95% by SDS-PAGE analysis

Theoretical Activity:15.6 U/mg Protein (r-SA:Biotin = 1:4(mol:mol))

Activity: ≥14 U/mg protein (Determined by NUPTEC according to modified Green Method)

Theoretical pI: 6.42

A280nm(1 mg/mL): 2.689

Storage Condition: -20 ℃

Storage Duration: 3 years

Description:

Streptavidin is a homotetrameric protein found in the culture broth of Streptomyces avidinii. Similar to avidin, one mole of streptavidin can bind 4 moles of biotin with a high affinity virtually unmatched in nature. Streptavidin lacks carbohydrate side chains present on avidin and has an isoelectric point close to neutrality. Therefore it has a reducing nonspecific binding level as compared to avidin. Streptavidin has been extensively applied in various biological fields, such as ELISA, IHC, TRFIA, quantifying PCR, isolation of single-stranded nucleotide, purification of biomolecule, and monoclonal antibody production as well.

M: Protein Molecular Weight Marker

Lane 1:Recombinant Streptavidin subunit

Lane 2:Recombinant Streptavidin homotetramer

For research use only!