Q NUPharose Fast Flow

Product Name:	Q NUPharose Fast Flow
Alternative Name:	Q NUPharose FF
Catalog Number:	NRPB22

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Description

Matrix ：6% Cross-linked agarose

Ligand ：Quaternary ammonium salt, -CH₂CH(OH)CH₂N(CH3)₃ +

Particle size range： 45~165 μm

Particle size, d50 ：~90 μm

Ionic capacity：110~160 μmol OH- /mL

Dynamic binding capacity, DBC_10% ：≥40 mg/mL^b ，≥110 mg/mL^c

Recommended operating flow velocity ：150~300 cm/h

Maximum flow velocity^e：1200 cm/h

pH stability ：3~12 (Recommended operating, pH), 3~12 (Long-term)；2~14 (CIP)

Chemical stability ：Stable to common aqueous buffers, 1.0 M NaOH, 8 M Urea, 6 M guanidine hydrochloride, 30% isopropanol and 70% ethanol

Storage & Shipping ：20% ethanol, 2~30 ℃

Shelf life ：5 years

Note: ^aMicrospheres accounting for more than 90% of the volume are in this particle size range; ^b DBC10% at 10% breakthrough for bovine serum albumin with 2 min residence time; ^c DBC10% at 10% breakthrough for Recombinant Human Epidermal Growth Factor (rh-EGF, NUPTEC, NRPA11S) with 2 min residence time; ^d 2 mg/mL bovine serum albumin incubated for 2 h at 25 °C; ^e 1 cm diameter column and 10 cm bed height using buffers with the same viscosity as water at 20 °C.