CM NUPharose Fast Flow

Product Name:	CM NUPharose Fast Flow
Alternative Name:	CM NUPharose FF
Catalog Number:	NRPB23

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Description

Matrix ：6% Cross-linked agarose

Ligand ：Carboxymethyl, -CH₂COO

Particle range^a： 45~165 μm

Particle size, d50 ：~90 μm

Dynamic binding capacity, DBC_10% ^b ：≥90 mg/mL

Ionic capacity：90~130 μmol H+ /mL

Recommended operating flow velocity ：150~300 cm/h

Maximum flow velocity^e：1200 cm/h

pH stability ：6~10（Recommended operating, pH），4~13（Long-term）；3~14（CIP）

Chemical stability ：Stable to common aqueous buffers, 1.0 M NaOH, 8 M Urea, 6 M guanidine hydrochloride, 30% isopropanol and 70% ethanol

Storage & Shipping ：20% ethanol，2 ℃~30 ℃

Shelf life ：5 years

Note: ^a Microspheres accounting for more than 90% of the volume are in this particle size range; ^b DBC10% at 10% breakthrough for lysozyme with 2 min residence time; ^c 1 cm diameter column and 10 cm bed height using buffers with the same viscosity as water at 20 °C.